BAIT
TBX5
HOS
T-box 5
GO Process (20)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- apoptotic nuclear changes [IDA]
- cardiac left ventricle formation [ISS]
- cell migration involved in coronary vasculogenesis [TAS]
- cell-cell signaling [IDA]
- embryonic forelimb morphogenesis [IMP]
- embryonic limb morphogenesis [IMP]
- forelimb morphogenesis [IMP]
- gene expression [TAS]
- heart development [IDA, IMP]
- negative regulation of cardiac muscle cell proliferation [IDA]
- negative regulation of cell migration [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of epithelial to mesenchymal transition [TAS]
- pericardium development [IDA]
- positive regulation of cardioblast differentiation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of execution phase of apoptosis [IC]
- transcription initiation from RNA polymerase II promoter [TAS]
- ventricular septum development [ISS]
Gene Ontology Molecular Function
Homo sapiens
PREY
DNAJA3
HCA57, TID1, hTID-1
DnaJ (Hsp40) homolog, subfamily A, member 3
GO Process (18)
GO Function (7)
GO Component (13)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- mitochondrion organization [IBA]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of apoptotic process [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- negative regulation of interferon-gamma-mediated signaling pathway [IDA]
- negative regulation of protein kinase activity [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- neuromuscular junction development [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- protein folding [IDA]
- protein refolding [IBA]
- protein stabilization [IDA]
- response to interferon-gamma [IDA]
- skeletal muscle acetylcholine-gated channel clustering [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- I-kappaB/NF-kappaB complex [IDA]
- IkappaB kinase complex [IDA]
- actin filament [IDA]
- cytoplasm [IDA]
- cytosol [IMP]
- extrinsic component of plasma membrane [ISS]
- intracellular membrane-bounded organelle [IDA]
- mitochondrial matrix [IDA]
- mitochondrial nucleoid [IDA]
- mitochondrion [IDA]
- neuromuscular junction [ISS]
- nucleus [IDA]
- postsynaptic membrane [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transcription factor protein interactomes reveal genetic determinants in heart disease.
Congenital heart disease (CHD) is present in 1% of live births, yet identification of causal mutations remains challenging. We hypothesized that genetic determinants for CHDs may lie in the protein interactomes of transcription factors whose mutations cause CHDs. Defining the interactomes of two transcription factors haplo-insufficient in CHD, GATA4 and TBX5, within human cardiac progenitors, and integrating the results with ... [more]
Cell Dec. 03, 2021; 185(5);794-814.e30 [Pubmed: 35182466]
Throughput
- High Throughput
Additional Notes
- Experiment carried out in HEK293 cells
Curated By
- BioGRID