Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Claudin-4 localization in epithelial ovarian cancer.

Neville MC, Webb PG, Baumgartner HK, Bitler BG

Claudin-4, a protein with the structure of classic claudins most often found in cell-cell junctions, is frequently overexpressed in epithelial cancers where its localization has not been studied. In this study we aimed to find out where this membrane protein is localized in an ovarian tumor model, OVCAR3 cells, that express high levels of the protein. Immunohistochemical studies showed claudin-4 ... [more]

Heliyon Oct. 01, 2022; 8(10);e10862 [Pubmed: 36237976]

Throughput

  • Low Throughput

Additional Notes

  • BioID
  • Experiments carried out in OVCAR3 and OVCAR8 cells

Curated By

  • BioGRID