Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

USP10 deubiquitinates Tau, mediating its aggregation.

Wei Z, Zeng K, Hu J, Li X, Huang F, Zhang B, Wang JZ, Liu R, Li HL, Wang X

Normal Tau promotes the assembly and stabilization of microtubules, thus, maintaining axon transport. In Alzheimer's disease (AD), Tau aggregation causes it to lose these above-mentioned functions. However, the molecular mechanism leading to Tau aggregation in AD remains ambiguous. Here, we report that USP10, one of the important deubiquitinases (DUBs), is involved in Tau aggregation. We found that USP10 is upregulated ... [more]

Cell Death Dis Aug. 20, 2022; 13(8);726 [Pubmed: 35987808]

Throughput

  • Low Throughput

Curated By

  • BioGRID