BAIT
HSPA8
HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
heat shock 70kDa protein 8
GO Process (14)
GO Function (11)
GO Component (13)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- RNA metabolic process [TAS]
- axon guidance [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- negative regulation of fibril organization [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neurotransmitter secretion [TAS]
- post-Golgi vesicle-mediated transport [TAS]
- protein folding [NAS]
- protein refolding [IDA]
- response to unfolded protein [NAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [NAS]
- G-protein coupled receptor binding [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA]
- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [NAS]
- G-protein coupled receptor binding [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA]
Gene Ontology Cellular Component
- Prp19 complex [IDA]
- blood microparticle [IDA]
- clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- intracellular [NAS]
- membrane [IDA]
- nucleus [IDA]
- plasma membrane [TAS]
- ribonucleoprotein complex [IDA]
- ubiquitin ligase complex [IDA]
Homo sapiens
PREY
IQGAP1
HUMORFA01, SAR1, p195
IQ motif containing GTPase activating protein 1
GO Process (13)
GO Function (9)
GO Component (17)
Gene Ontology Biological Process
- cellular response to calcium ion [IDA]
- cellular response to epidermal growth factor stimulus [IMP]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [IMP]
- glomerular visceral epithelial cell development [ISS]
- negative regulation of catalytic activity [TAS]
- neuron projection extension [IMP]
- positive regulation of GTPase activity [TAS]
- positive regulation of protein kinase activity [IMP]
- positive regulation of protein serine/threonine kinase activity [IDA]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
Gene Ontology Cellular Component
- actin cytoskeleton [ISS]
- actin filament [TAS]
- axon [ISS]
- cell junction [IDA]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- focal adhesion [IDA]
- growth cone [ISS]
- microtubule [IDA]
- microtubule cytoskeleton [ISS]
- midbody [IDA]
- neuron projection [ISS]
- nucleoplasm [IDA]
- plasma membrane [IDA, TAS]
- slit diaphragm [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteome-wide identification of HSP70/HSC70 chaperone clients in human cells.
The 70 kDa heat shock protein (HSP70) family of chaperones are the front line of protection from stress-induced misfolding and aggregation of polypeptides in most organisms and are responsible for promoting the stability, folding, and degradation of clients to maintain cellular protein homeostasis. Here, we demonstrate quantitative identification of HSP70 and 71 kDa heat shock cognate (HSC70) clients using a ... [more]
PLoS Biol Dec. 01, 2019; 18(7);e3000606 [Pubmed: 32687490]
Throughput
- High Throughput
Additional Notes
- Affinity capture MS was carried out to identify high confidence protein interactors
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HSPA8 IQGAP1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3437120 |
Curated By
- BioGRID