BAIT
HSPA8
HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71
heat shock 70kDa protein 8
GO Process (14)
GO Function (11)
GO Component (13)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- RNA metabolic process [TAS]
- axon guidance [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- membrane organization [TAS]
- negative regulation of fibril organization [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neurotransmitter secretion [TAS]
- post-Golgi vesicle-mediated transport [TAS]
- protein folding [NAS]
- protein refolding [IDA]
- response to unfolded protein [NAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [NAS]
- G-protein coupled receptor binding [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA]
- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [NAS]
- G-protein coupled receptor binding [IPI]
- MHC class II protein complex binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA]
Gene Ontology Cellular Component
- Prp19 complex [IDA]
- blood microparticle [IDA]
- clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- intracellular [NAS]
- membrane [IDA]
- nucleus [IDA]
- plasma membrane [TAS]
- ribonucleoprotein complex [IDA]
- ubiquitin ligase complex [IDA]
Homo sapiens
PREY
PTPN2
PTN2, PTPT, TC-PTP, TCELLPTP, TCPTP
protein tyrosine phosphatase, non-receptor type 2
GO Process (34)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- B cell differentiation [ISS]
- T cell differentiation [ISS]
- cytokine-mediated signaling pathway [TAS]
- erythrocyte differentiation [ISS]
- glucose homeostasis [ISS]
- insulin receptor signaling pathway [ISS]
- interferon-gamma-mediated signaling pathway [TAS]
- negative regulation of ERK1 and ERK2 cascade [ISS]
- negative regulation of T cell receptor signaling pathway [ISS]
- negative regulation of cell proliferation [IMP]
- negative regulation of chemotaxis [ISS]
- negative regulation of epidermal growth factor receptor signaling pathway [IMP]
- negative regulation of inflammatory response [ISS]
- negative regulation of insulin receptor signaling pathway [ISS]
- negative regulation of interferon-gamma-mediated signaling pathway [ISS]
- negative regulation of interleukin-2-mediated signaling pathway [IMP]
- negative regulation of interleukin-4-mediated signaling pathway [IMP]
- negative regulation of interleukin-6-mediated signaling pathway [IMP]
- negative regulation of lipid storage [ISS]
- negative regulation of macrophage colony-stimulating factor signaling pathway [ISS]
- negative regulation of macrophage differentiation [ISS]
- negative regulation of platelet-derived growth factor receptor-beta signaling pathway [ISS]
- negative regulation of positive thymic T cell selection [ISS]
- negative regulation of prolactin signaling pathway [ISS]
- negative regulation of tumor necrosis factor-mediated signaling pathway [ISS]
- negative regulation of type I interferon-mediated signaling pathway [IMP]
- negative regulation of tyrosine phosphorylation of Stat1 protein [IDA, IMP]
- negative regulation of tyrosine phosphorylation of Stat3 protein [IDA]
- negative regulation of tyrosine phosphorylation of Stat5 protein [ISS]
- negative regulation of tyrosine phosphorylation of Stat6 protein [IMP]
- peptidyl-tyrosine dephosphorylation [IDA, IMP]
- positive regulation of gluconeogenesis [ISS]
- regulation of hepatocyte growth factor receptor signaling pathway [IMP]
- regulation of interferon-gamma-mediated signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteome-wide identification of HSP70/HSC70 chaperone clients in human cells.
The 70 kDa heat shock protein (HSP70) family of chaperones are the front line of protection from stress-induced misfolding and aggregation of polypeptides in most organisms and are responsible for promoting the stability, folding, and degradation of clients to maintain cellular protein homeostasis. Here, we demonstrate quantitative identification of HSP70 and 71 kDa heat shock cognate (HSC70) clients using a ... [more]
PLoS Biol Dec. 01, 2019; 18(7);e3000606 [Pubmed: 32687490]
Throughput
- High Throughput
Additional Notes
- Affinity capture MS was carried out to identify high confidence protein interactors
Curated By
- BioGRID