Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The parafibromin tumor suppressor protein interacts with actin-binding proteins actinin-2 and actinin-3.

Agarwal SK, Simonds WF, Marx SJ

BACKGROUND: Germline and somatic inactivating mutations in the HRPT2 gene occur in the inherited hyperparathyroidism-jaw tumor syndrome, in some cases of parathyroid cancer and in some cases of familial hyperparathyroidism. HRPT2 encodes parafibromin. To identify parafibromin interacting proteins we used the yeast two-hybrid system for screening a heart cDNA library with parafibromin as the bait. RESULTS: Fourteen parafibromin interaction positive ... [more]

Mol. Cancer Aug. 09, 2008; 7(0);65 [Pubmed: 18687124]

Throughput

  • Low Throughput

Curated By

  • BioGRID