BAIT
HSPA1A
HEL-S-103, HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, DAQB-147D11.1
heat shock 70kDa protein 1A
GO Process (20)
GO Function (13)
GO Component (17)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- RNA metabolic process [TAS]
- cellular heat acclimation [IMP]
- cellular response to heat [IDA]
- cellular response to oxidative stress [TAS]
- gene expression [TAS]
- mRNA catabolic process [IDA]
- mRNA metabolic process [TAS]
- negative regulation of apoptotic process [IMP, TAS]
- negative regulation of cell death [IDA, IMP]
- negative regulation of cell growth [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [IMP]
- negative regulation of inclusion body assembly [IDA]
- negative regulation of protein ubiquitination [IDA]
- positive regulation of erythrocyte differentiation [IMP]
- protein refolding [IDA]
- protein stabilization [TAS]
- regulation of cell death [IMP]
- response to unfolded protein [IDA]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [IDA]
- G-protein coupled receptor binding [IPI]
- double-stranded RNA binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein binding involved in protein folding [IDA]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA, NAS, TAS]
- ATP binding [IDA]
- ATPase activity [IDA]
- ATPase activity, coupled [IDA]
- G-protein coupled receptor binding [IPI]
- double-stranded RNA binding [IDA]
- enzyme binding [IPI]
- heat shock protein binding [IPI]
- poly(A) RNA binding [IDA]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein binding involved in protein folding [IDA]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IDA, NAS, TAS]
Gene Ontology Cellular Component
- COP9 signalosome [IDA]
- aggresome [IDA]
- blood microparticle [IDA]
- centriole [IDA]
- cytoplasm [IDA, TAS]
- cytosol [IDA, TAS]
- endoplasmic reticulum [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- inclusion body [IDA]
- mitochondrion [TAS]
- nuclear speck [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- ribonucleoprotein complex [IDA]
- ubiquitin ligase complex [IDA]
- vesicle [IDA]
Homo sapiens
PREY
BAX
BCL2L4
BCL2-associated X protein
GO Process (36)
GO Function (7)
GO Component (13)
Gene Ontology Biological Process
- B cell apoptotic process [IDA]
- B cell receptor apoptotic signaling pathway [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c [IDA]
- apoptotic mitochondrial changes [IDA]
- apoptotic process [NAS, TAS]
- apoptotic signaling pathway [IDA]
- endoplasmic reticulum calcium ion homeostasis [TAS]
- establishment or maintenance of transmembrane electrochemical gradient [IDA]
- extrinsic apoptotic signaling pathway [IDA]
- extrinsic apoptotic signaling pathway in absence of ligand [IBA]
- extrinsic apoptotic signaling pathway via death domain receptors [IC]
- intrinsic apoptotic signaling pathway [IDA, TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IBA]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IMP]
- mitochondrial fragmentation involved in apoptotic process [IDA]
- mitochondrial fusion [IDA]
- negative regulation of protein binding [IDA]
- positive regulation of apoptotic DNA fragmentation [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of endoplasmic reticulum unfolded protein response [IMP]
- positive regulation of intrinsic apoptotic signaling pathway [IMP]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [TAS]
- positive regulation of neuron apoptotic process [IDA]
- positive regulation of protein oligomerization [IDA]
- positive regulation of release of cytochrome c from mitochondria [IDA]
- protein homooligomerization [IDA]
- protein oligomerization [IDA]
- regulation of mitochondrial membrane potential [IDA]
- regulation of protein heterodimerization activity [IPI]
- regulation of protein homodimerization activity [IDA]
- release of cytochrome c from mitochondria [IDA]
- release of matrix enzymes from mitochondria [IDA]
- response to toxic substance [IDA]
- retinal cell apoptotic process [IMP]
- transformed cell apoptotic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- BAX complex [IDA]
- Bcl-2 family protein complex [IDA]
- cytosol [IDA, TAS]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum membrane [IDA]
- extracellular vesicular exosome [IDA]
- membrane [IDA]
- mitochondrial outer membrane [IBA, TAS]
- mitochondrial permeability transition pore complex [IDA]
- mitochondrion [IDA]
- nuclear envelope [IDA]
- nucleus [IDA, IMP]
- pore complex [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proteome-wide identification of HSP70/HSC70 chaperone clients in human cells.
The 70 kDa heat shock protein (HSP70) family of chaperones are the front line of protection from stress-induced misfolding and aggregation of polypeptides in most organisms and are responsible for promoting the stability, folding, and degradation of clients to maintain cellular protein homeostasis. Here, we demonstrate quantitative identification of HSP70 and 71 kDa heat shock cognate (HSC70) clients using a ... [more]
PLoS Biol Dec. 01, 2019; 18(7);e3000606 [Pubmed: 32687490]
Throughput
- High Throughput
Additional Notes
- Proximity label MS was carried out to identify high confidence protein interactors with a FDR cutoff of 0.05
Curated By
- BioGRID