An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Identification of proteins that interact with alpha A-crystallin using a human proteome microarray.

Fan Q, Huang LZ, Zhu XJ, Zhang KK, Ye HF, Luo Y, Sun XH, Zhou P, Lu Y

To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray.The active full-length CRYAA protein corresponding to amino acids 1-173 of CRYAA was recombined. A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used ... [more]

Mol Vis Jan. 24, 2014; 20();117-24 [Pubmed: 24453475]

Throughput

High Throughput

Additional Notes

Human proteome microarray

Curated By

BioGRID

Interaction Summary

CRYAA

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]unfolded protein binding [IMP, IPI]

Gene Ontology Cellular Component

TGOLN2