Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Identification of proteins that interact with alpha A-crystallin using a human proteome microarray.

Fan Q, Huang LZ, Zhu XJ, Zhang KK, Ye HF, Luo Y, Sun XH, Zhou P, Lu Y

To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray.The active full-length CRYAA protein corresponding to amino acids 1-173 of CRYAA was recombined. A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used ... [more]

Mol Vis Jan. 24, 2014; 20();117-24 [Pubmed: 24453475]

Throughput

  • High Throughput

Additional Notes

  • Human proteome microarray

Curated By

  • BioGRID