BAIT

NSP12

ORF1ab, ORF1ab-nsp12, SARS-CoV2 nsp12, SARS-CoV-2 nsp12, RNA-directed RNA polymerase, R1AB_SARS2, PRO_0000449629, GU280_gp01_nsp12
RNA-directed RNA polymerase; Non-structural protein 12
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

ZC3H7A

ZC3H7, ZC3HDC7, HSPC055
zinc finger CCCH-type containing 7A
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Global landscape of the host response to SARS-CoV-2 variants reveals viral evolutionary trajectories

Bouhaddou M, Reuschl A-K, Polacco BJ, Thorne LG, Ummadi MR, Ye C, Ramirez RR, Pelin A, Batra J, Jang GM, Xu J, Moen JM, Richards AL, Zhou Y, Harjai B, Stevenson E, Rojc A, Ragazzini R, Whelan MVX, Furnon W, De Lorenzo G, Cowton V, Syed AM, Ciling A, Deutsch N, Pirak D, Dowgier G, Mesner D, Turner JL, McGovern BL, Rodriguez ML, Leiva-Rebollo R, Dunham AS, Zhong X, Eckhardt M, Fossati A, Liotta N, Kehrer T, Cupic A, Rutkowska M, Mena N, Aslam S, Hoffert A, Foussard H, Pham J, Ly

A series of SARS-CoV-2 variants of concern (VOCs) have evolved in humans during the COVID-19 pandemic--Alpha, Beta, Gamma, Delta, and Omicron. Here, we used global proteomic and genomic analyses during infection to understand the molecular responses driving VOC evolution. We discovered VOC-specific differences in viral RNA and protein expression levels, including for N, Orf6, and Orf9b, and pinpointed several viral ... [more]

Status: Preliminary Report

Throughput

  • High Throughput

Additional Notes

  • SARS-CoV-2 alpha_beta_delta_gamma_omicron variant (Protein Mutation: nsp12__P314L)
  • SARS-CoV-2 cal20c variant (Protein Mutation: nsp12__P324L)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP12 ZC3H7A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9808BioGRID
2652420
NSP12 ZC3H7A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9808BioGRID
2875369
NSP12 ZC3H7A
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2799070

Curated By

  • BioGRID