BAIT

URA1

dihydroorotate dehydrogenase, L000002430, YKL216W
Dihydroorotate dehydrogenase; catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

FRD1

FRDS1, YEL047C
Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
GO Process (1)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Multiple knockout analysis of genetic robustness in the yeast metabolic network.

Deutscher D, Meilijson I, Kupiec M, Ruppin E

Genetic robustness characterizes the constancy of the phenotype in face of heritable perturbations. Previous investigations have used comprehensive single and double gene knockouts to study gene essentiality and pairwise gene interactions in the yeast Saccharomyces cerevisiae. Here we conduct an in silico multiple knockout investigation of a flux balance analysis model of the yeast's metabolic network. Cataloging gene sets that ... [more]

Nat. Genet. Sep. 01, 2006; 38(9);993-8 [Pubmed: 16941010]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • COX1/DIC1/URA1/YEL047C quadruple mutant is synthetic lethal in synthetic rich media
  • DIC1/RIP1/URA1/YEL047C quadruple mutant is synthetic lethal in synthetic rich media
  • FUM1/RIP1/URA1/YEL047C quadruple mutant is synthetic lethal in synthetic rich media
  • genetic complex

Curated By

  • BioGRID