A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Multiple knockout analysis of genetic robustness in the yeast metabolic network.

Deutscher D, Meilijson I, Kupiec M, Ruppin E

Genetic robustness characterizes the constancy of the phenotype in face of heritable perturbations. Previous investigations have used comprehensive single and double gene knockouts to study gene essentiality and pairwise gene interactions in the yeast Saccharomyces cerevisiae. Here we conduct an in silico multiple knockout investigation of a flux balance analysis model of the yeast's metabolic network. Cataloging gene sets that ... [more]

Nat. Genet. Sep. 01, 2006; 38(9);993-8 [Pubmed: 16941010]

Throughput

High Throughput

Ontology Terms

inviable (APO:0000112)

Additional Notes

double mutants are synthetic lethal in minimal media
genetic complex

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MET10 MET5	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	10	BioGRID	3609137

Curated By

BioGRID

Interaction Summary

MET5

Gene Ontology Biological Process

Gene Ontology Molecular Function

sulfite reductase (NADPH) activity [IDA]

Gene Ontology Cellular Component

MET10