PREY

PIAS2

ARIP3, DIP, MIZ, MIZ1, PIASX, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4
protein inhibitor of activated STAT, 2
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A crosstalk between hSiah2 and Pias E3-ligases modulates Pias-dependent activation.

Depaux A, Regnier-Ricard F, Germani A, Varin-Blank N

Protein inhibitor of activated STAT (Pias) and human homologues of seven in absentia (hSiah) proteins both exhibit properties of ubiquitin-family peptides conjugating enzymes. Pias present E3-ligase activity for small ubiquitin-related modifiers (Sumo) covalent attachment to their targets. This post-translational modification is responsible for the activation of different transcription factors such as AP1. HSiah proteins possess ubiquitin-E3-ligase activity that triggers their ... [more]

Oncogene Oct. 11, 2007; 26(46);6665-76 [Pubmed: 17533377]

Throughput

  • Low Throughput

Curated By

  • BioGRID