BAIT

TPM1

tropomyosin TPM1, L000002328, YNL079C
Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

BUD14

protein phosphatase regulator BUD14, YAR014C
Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Displacement of formins from growing barbed ends by bud14 is critical for actin cable architecture and function.

Chesarone M, Gould CJ, Moseley JB, Goode BL

Normal cellular development and function require tight spatiotemporal control of actin assembly. Formins are potent actin assembly factors that protect the growing ends of actin filaments from capping proteins. However, it is unresolved how the duration of formin-mediated actin assembly events is controlled, whether formins are actively displaced from growing ends, and how filament length is regulated in vivo. Here, ... [more]

Dev. Cell Feb. 01, 2009; 16(2);292-302 [Pubmed: 19217430]

Throughput

  • Low Throughput

Ontology Terms

  • viability (APO:0000111)

Additional Notes

  • deletion of BUD14 rescues the growth defect of TPM1 mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TPM1 BUD14
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3237BioGRID
2168468

Curated By

  • BioGRID