ISG15
Gene Ontology Biological Process
- ISG15-protein conjugation [IDA, IMP]
- cytokine-mediated signaling pathway [TAS]
- defense response to bacterium [IMP]
- defense response to virus [IMP]
- innate immune response [TAS]
- negative regulation of protein ubiquitination [IDA]
- negative regulation of type I interferon production [TAS]
- negative regulation of viral genome replication [IMP]
- regulation of interferon-gamma production [IMP]
- response to type I interferon [IDA]
- type I interferon signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
USP14
Gene Ontology Biological Process
- negative regulation of ER-associated ubiquitin-dependent protein catabolic process [IMP]
- negative regulation of endopeptidase activity [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA]
- regulation of chemotaxis [IMP]
- regulation of proteasomal protein catabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Expedient Synthesis of Ubiquitin-like Protein ISG15 Tools through Chemo-Enzymatic Ligation Catalyzed by a Viral Protease Lbpro.
Ubiquitin (Ub)-like protein ISG15 (interferon-stimulated gene 15) regulates innate immunity and links with the evasion of host response by viruses such as SARS-CoV-2. Dissecting ISGylation pathways recently received increasing attention which can inform related disease interventions, but such studies necessitate the preparation and development of various ISG15 protein tools. Here, we find that the leader protease (Lbpro ) encoded by ... [more]
Throughput
- High Throughput
Additional Notes
- #LLPI
- Likely protein-protein interaction
- Pull down (reconstituted complex) - MS was carried out to identify high confidence protein interactors with a P value <0.01 and fold enrichment (average log2)>3
Curated By
- BioGRID