Publication

Expedient Synthesis of Ubiquitin-like Protein ISG15 Tools through Chemo-Enzymatic Ligation Catalyzed by a Viral Protease Lbpro.

Wang T, Li C, Wang M, Zhang J, Zheng Q, Liang L, Chu G, Tian X, Deng H, He W, Liu L, Li J

Ubiquitin (Ub)-like protein ISG15 (interferon-stimulated gene 15) regulates innate immunity and links with the evasion of host response by viruses such as SARS-CoV-2. Dissecting ISGylation pathways recently received increasing attention which can inform related disease interventions, but such studies necessitate the preparation and development of various ISG15 protein tools. Here, we find that the leader protease (Lbpro ) encoded by ... [more]

Throughput

• High Throughput

Additional Notes

• #LLPI
• Likely protein-protein interaction
• Pull down (reconstituted complex) - MS was carried out to identify high confidence protein interactors with a P value <0.01 and fold enrichment (average log2)>3

Curated By

• BioGRID