CD63
Gene Ontology Biological Process
- blood coagulation [TAS]
- cell migration [IMP]
- cell-matrix adhesion [IMP]
- cellular protein localization [IDA]
- endosome to melanosome transport [IMP]
- pigment granule maturation [IMP]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of integrin-mediated signaling pathway [IMP]
- positive regulation of receptor internalization [IMP]
- regulation of rubidium ion transport [IDA]
- regulation of vascular endothelial growth factor signaling pathway [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell surface [IDA]
- endosome lumen [IDA]
- endosome membrane [IDA]
- extracellular vesicular exosome [IDA]
- integral component of plasma membrane [TAS]
- intrinsic component of plasma membrane [IDA]
- late endosome membrane [IDA]
- lysosomal membrane [IDA]
- multivesicular body, internal vesicle [IDA]
- plasma membrane [TAS]
- platelet dense granule membrane [TAS]
SDCBP
Gene Ontology Biological Process
- actin cytoskeleton organization [NAS]
- axon guidance [TAS]
- intracellular signal transduction [NAS]
- positive regulation of JNK cascade [IC]
- positive regulation of exosomal secretion [IMP]
- positive regulation of extracellular vesicular exosome assembly [IMP]
- positive regulation of phosphorylation [IDA]
- protein targeting to membrane [NAS]
- substrate-dependent cell migration, cell extension [NAS]
- synaptic transmission [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Chloroquine treatment induces secretion of autophagy-related proteins and inclusion of Atg8-family proteins in distinct extracellular vesicle populations.
Chloroquine (CQ), a lysosomotropic agent, is commonly used to inhibit lysosomal degradation and macroautophagy/autophagy. Here we investigated the cell-extrinsic effects of CQ on secretion. We showed that lysosomal and autophagy inhibition by CQ altered the secretome, and induced the release of Atg8 orthologs and autophagy receptors. Atg8-family proteins, in particular, were secreted inside small extracellular vesicles (sEVs) in a lipidation-dependent ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CD63 SDCBP | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID