An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Physical and functional interactome atlas of human receptor tyrosine kinases.

Salokas K, Liu X, Oehman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M

Much cell-to-cell communication is facilitated by cell surface receptor tyrosine kinases (RTKs). These proteins phosphorylate their downstream cytoplasmic substrates in response to stimuli such as growth factors. Despite their central roles, the functions of many RTKs are still poorly understood. To resolve the lack of systematic knowledge, we apply three complementary methods to map the molecular context and substrate profiles ... [more]

EMBO Rep Jun. 07, 2022; 23(6);e54041 [Pubmed: 35384245]

Throughput

High Throughput

Additional Notes

Affinity capture MS was carried out to identify high confidence ligand induced protein interactors with a PSM of greater than of equal to 5

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FGFR1 HYOU1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Salokas K (2022)	High	0	BioGRID	3507780

Curated By

BioGRID

Interaction Summary

FGFR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

fibroblast growth factor binding [IDA]fibroblast growth factor-activated receptor activity [IDA, TAS]heparin binding [IDA]identical protein binding [IPI]protein binding [IPI]protein homodimerization activity [IPI]protein tyrosine kinase activity [IDA]

Gene Ontology Cellular Component

HYOU1