BAIT

EGFR

ERBB, ERBB1, HER1, NISBD2, PIG61, mENA
epidermal growth factor receptor
GO Process (42)
GO Function (15)
GO Component (13)

Gene Ontology Biological Process

Homo sapiens
PREY

TUBB2A

CDCBM5, TUBB, TUBB2, dJ40E16.7
tubulin, beta 2A class IIa
GO Process (3)
GO Function (0)
GO Component (4)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Physical and functional interactome atlas of human receptor tyrosine kinases.

Salokas K, Liu X, Oehman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M

Much cell-to-cell communication is facilitated by cell surface receptor tyrosine kinases (RTKs). These proteins phosphorylate their downstream cytoplasmic substrates in response to stimuli such as growth factors. Despite their central roles, the functions of many RTKs are still poorly understood. To resolve the lack of systematic knowledge, we apply three complementary methods to map the molecular context and substrate profiles ... [more]

EMBO Rep Jun. 07, 2022; 23(6);e54041 [Pubmed: 35384245]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EGFR TUBB2A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low/High-BioGRID
-
EGFR TUBB2A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.01BioGRID
3506516

Curated By

  • BioGRID