WDHD1
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RPA2
Gene Ontology Biological Process
- DNA recombinase assembly [TAS]
- DNA repair [TAS]
- DNA replication [IDA, IMP, TAS]
- DNA strand elongation involved in DNA replication [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- base-excision repair [IDA]
- double-strand break repair [TAS]
- double-strand break repair via homologous recombination [IMP, TAS]
- mismatch repair [IMP]
- mitotic G1 DNA damage checkpoint [IMP]
- mitotic cell cycle [TAS]
- nucleotide-excision repair [IMP, TAS]
- nucleotide-excision repair, DNA damage removal [TAS]
- nucleotide-excision repair, DNA gap filling [TAS]
- regulation of DNA damage checkpoint [IMP]
- regulation of double-strand break repair via homologous recombination [IMP]
- telomere maintenance [IMP, TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
And-1 coordinates with Claspin for efficient Chk1 activation in response to replication stress.
The replisome is important for DNA replication checkpoint activation, but how specific components of the replisome coordinate with ATR to activate Chk1 in human cells remains largely unknown. Here, we demonstrate that And-1, a replisome component, acts together with ATR to activate Chk1. And-1 is phosphorylated at T826 by ATR following replication stress, and this phosphorylation is required for And-1 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPA2 WDHD1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| RPA2 WDHD1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3632815 |
Curated By
- BioGRID