An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Restriction factor screening identifies RABGAP1L-mediated disruption of endocytosis as a host antiviral defense.

Fernbach S, Spieler EE, Busnadiego I, Karakus U, Lkharrazi A, Stertz S, Hale BG

Host interferons (IFNs) powerfully restrict viruses through the action of several hundred IFN-stimulated gene (ISG) products, many of which remain uncharacterized. Here, using RNAi screening, we identify several ISG restriction factors with previously undescribed contributions to IFN-mediated defense. Notably, RABGAP1L, a Tre2/Bub2/Cdc16 (TBC)-domain-containing protein involved in regulation of small membrane-bound GTPases, robustly potentiates IFN action against influenza A viruses (IAVs). ... [more]

Cell Rep Mar. 22, 2022; 38(12);110549 [Pubmed: 35320721]

Throughput

High Throughput|Low Throughput

Additional Notes

LTP hits identified by western

Curated By

BioGRID

Interaction Summary

RABGAP1L

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase activator activity [IDA]Rab GTPase binding [IDA]

Gene Ontology Cellular Component

VPS33A