BAIT

NSP3

ORF1ab, ORF1ab-nsp3, SARS-CoV2 nsp3, SARS-CoV-2 nsp3, R1AB_SARS2, PRO_0000449621, GU280_gp01_nsp3
Non-structural protein 3
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

CCZ1B

C7orf28B, H_NH0577018.2
CCZ1 vacuolar protein trafficking and biogenesis associated homolog B (S. cerevisiae)
GO Process (1)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

An antibody-based proximity labeling map reveals mechanisms of SARS-CoV-2 inhibition of antiviral immunity.

Zhang Y, Shang L, Zhang J, Liu Y, Jin C, Zhao Y, Lei X, Wang W, Xiao X, Zhang X, Liu Y, Liu L, Zhuang MW, Mi Q, Tian C, Wang J, He F, Wang PH, Wang J

The global epidemic caused by the coronavirus severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in the infection of over 200 million people. To extend the knowledge of interactions between SARS-CoV-2 and humans, we systematically investigate the interactome of 29 viral proteins in human cells by using an antibody-based TurboID assay. In total, 1,388 high-confidence human proximal proteins with biotinylated ... [more]

Cell Chem Biol Dec. 20, 2021; 29(1);5-18.e6 [Pubmed: 34672954]

Throughput

  • High Throughput

Additional Notes

  • An Nsp3 C-terminal fragment (NSP3C) was used in the experiment.
  • BioID
  • High confidence SARS-CoV-2 viral and human protein interactions were identified in HEK293T cells using the TurboID proximity labeling method.
  • TurboID

Curated By

  • BioGRID