BAIT

RAD51D

BROVCA4, R51H3, RAD51L3, TRAD
RAD51 paralog D
Homo sapiens
PREY

ANXA3

ANX3
annexin A3
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A protein interaction landscape of breast cancer.

Kim M, Park J, Bouhaddou M, Kim K, Rojc A, Modak M, Soucheray M, McGregor MJ, O'Leary P, Wolf D, Stevenson E, Foo TK, Mitchell D, Herrington KA, Munoz DP, Tutuncuoglu B, Chen KH, Zheng F, Kreisberg JF, Diolaiti ME, Gordan JD, Coppe JP, Swaney DL, Xia B, van 't Veer L, Ashworth A, Ideker T, Krogan NJ

Cancers have been associated with a diverse array of genomic alterations. To help mechanistically understand such alterations in breast-invasive carcinoma, we applied affinity purification–mass spectrometry to delineate comprehensive biophysical interaction networks for 40 frequently altered breast cancer (BC) proteins, with and without relevant mutations, across three human breast cell lines. These networks identify cancer-specific protein-protein interactions (PPIs), interconnected and enriched ... [more]

Science Oct. 01, 2021; 374(6563);eabf3066 [Pubmed: 34591612]

Throughput

  • Low Throughput

Curated By

  • BioGRID