An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A protein interaction landscape of breast cancer.

Kim M, Park J, Bouhaddou M, Kim K, Rojc A, Modak M, Soucheray M, McGregor MJ, O'Leary P, Wolf D, Stevenson E, Foo TK, Mitchell D, Herrington KA, Munoz DP, Tutuncuoglu B, Chen KH, Zheng F, Kreisberg JF, Diolaiti ME, Gordan JD, Coppe JP, Swaney DL, Xia B, van 't Veer L, Ashworth A, Ideker T, Krogan NJ

Cancers have been associated with a diverse array of genomic alterations. To help mechanistically understand such alterations in breast-invasive carcinoma, we applied affinity purification–mass spectrometry to delineate comprehensive biophysical interaction networks for 40 frequently altered breast cancer (BC) proteins, with and without relevant mutations, across three human breast cell lines. These networks identify cancer-specific protein-protein interactions (PPIs), interconnected and enriched ... [more]

Science Oct. 01, 2021; 374(6563);eabf3066 [Pubmed: 34591612]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MLH1 SPTBN2	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.1203	BioGRID	1272520

Curated By

BioGRID

Interaction Summary

MLH1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IBA]MutSalpha complex binding [IDA]protein binding [IPI]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

SPTBN2