TOP2A
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA ligation [IDA]
- DNA topological change [IDA]
- DNA unwinding involved in DNA replication [IBA]
- apoptotic chromosome condensation [IDA]
- cellular response to DNA damage stimulus [IDA]
- chromosome segregation [IMP]
- mitotic DNA integrity checkpoint [IBA]
- mitotic cell cycle [TAS]
- mitotic recombination [IBA]
- positive regulation of apoptotic process [IDA]
- positive regulation of single stranded viral RNA replication via double stranded DNA intermediate [IMP]
- positive regulation of viral genome replication [IMP]
- resolution of meiotic recombination intermediates [IBA]
- sister chromatid segregation [IBA]
Gene Ontology Molecular Function- DNA binding [IDA]
- DNA binding, bending [IDA]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA]
- DNA-dependent ATPase activity [IDA]
- chromatin binding [IDA]
- drug binding [IDA]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- magnesium ion binding [IDA]
- poly(A) RNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- protein kinase C binding [IPI]
- ubiquitin binding [IMP]
- DNA binding [IDA]
- DNA binding, bending [IDA]
- DNA topoisomerase type II (ATP-hydrolyzing) activity [IDA]
- DNA-dependent ATPase activity [IDA]
- chromatin binding [IDA]
- drug binding [IDA]
- enzyme binding [IPI]
- histone deacetylase binding [IPI]
- magnesium ion binding [IDA]
- poly(A) RNA binding [IDA]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- protein kinase C binding [IPI]
- ubiquitin binding [IMP]
Gene Ontology Cellular Component
USP15
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- histone H2B conserved C-terminal lysine deubiquitination [IDA]
- monoubiquitinated protein deubiquitination [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- pathway-restricted SMAD protein phosphorylation [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein deubiquitination [IDA]
- regulation of proteasomal protein catabolic process [IBA]
- transforming growth factor beta receptor signaling pathway [IDA, IMP, TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
GINS1 promotes the proliferation and migration of glioma cells through USP15-mediated deubiquitination of TOP2A.
GINS1 is a GINS complex subunit that functions along with the MCM2-7 complex and Cdc45 in eukaryotic DNA replication. Despite the significance of the GINS complex in the switch between quiescence and proliferation of glioma cells inside and outside the perinecrotic niche, the biological functions and the underlying mechanism of GINS1 remain unclear. Unlike in normal cells and tissues, GINS1 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| USP15 TOP2A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID