BAIT
BCKDK
AI327402
branched chain ketoacid dehydrogenase kinase
GO Process (3)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
DNAJA3
1200003J13Rik, 1810053A11Rik, C81173, Tid-1, Tid1l
DnaJ (Hsp40) homolog, subfamily A, member 3
GO Process (29)
GO Function (10)
GO Component (13)
Gene Ontology Biological Process
- T cell differentiation in thymus [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- activation-induced cell death of T cells [IMP]
- apoptotic process [IMP]
- cell aging [IDA]
- embryo development [IMP]
- mitochondrial DNA replication [IMP]
- mitochondrion organization [IMP]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- negative regulation of NF-kappaB transcription factor activity [ISA, ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of interferon-gamma-mediated signaling pathway [ISO]
- negative regulation of programmed cell death [IMP]
- negative regulation of protein kinase activity [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- neuromuscular junction development [IMP, ISO]
- positive regulation of T cell proliferation [IMP]
- positive regulation of apoptotic process [ISO]
- positive regulation of protein ubiquitination [ISO]
- protein folding [ISO]
- protein refolding [IBA]
- protein stabilization [ISO]
- regulation of apoptotic process [IBA]
- regulation of catalytic activity [IDA]
- response to interferon-gamma [ISO]
- skeletal muscle acetylcholine-gated channel clustering [IMP]
- small GTPase mediated signal transduction [IDA]
Gene Ontology Molecular Function- Hsp70 protein binding [ISO]
- NF-kappaB binding [ISO]
- chaperone binding [IBA]
- interferon-gamma receptor binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor tyrosine kinase binding [IPI]
- small GTPase regulator activity [IDA]
- transcription factor binding [ISO]
- unfolded protein binding [IPI]
- Hsp70 protein binding [ISO]
- NF-kappaB binding [ISO]
- chaperone binding [IBA]
- interferon-gamma receptor binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor tyrosine kinase binding [IPI]
- small GTPase regulator activity [IDA]
- transcription factor binding [ISO]
- unfolded protein binding [IPI]
Gene Ontology Cellular Component
- I-kappaB/NF-kappaB complex [ISO]
- IkappaB kinase complex [ISO]
- actin filament [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- extrinsic component of plasma membrane [IDA]
- intracellular membrane-bounded organelle [ISO]
- mitochondrial matrix [ISO]
- mitochondrial nucleoid [ISO]
- mitochondrion [IDA, IMP, ISO]
- neuromuscular junction [IDA]
- nucleus [IDA, ISO]
- postsynaptic membrane [IDA]
Mus musculus
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.
There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]
Cell Rep Nov. 22, 2022; 41(8);111678 [Pubmed: 36417873]
Quantitative Score
- 0.493263604 [Relative Biotinylation Score]
Throughput
- High Throughput
Additional Notes
- BioID experiment
- The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
- The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control
Curated By
- BioGRID