BAIT
BRSK2
4833424K13Rik, SAD-A, SADA
BR serine/threonine kinase 2
GO Process (10)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [ISO]
- actin cytoskeleton reorganization [ISO]
- axonogenesis [IMP]
- establishment of cell polarity [IGI, IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISO]
- neuron differentiation [IGI]
- neuron projection morphogenesis [IGI]
- peptidyl-serine phosphorylation [IDA]
- protein phosphorylation [IDA, ISO, TAS]
- regulation of insulin secretion involved in cellular response to glucose stimulus [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
NPM1
B23, NO38, Npm, RP23-323L8.2
nucleophosmin 1
GO Process (39)
GO Function (18)
GO Component (16)
Gene Ontology Biological Process
- DNA repair [ISO]
- cell aging [ISO]
- cell growth [IDA]
- cell volume homeostasis [IDA, IMP]
- centrosome cycle [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of centrosome duplication [ISO]
- negative regulation of mRNA splicing, via spliceosome [IDA]
- negative regulation of protein kinase activity by regulation of protein phosphorylation [ISO]
- nucleocytoplasmic transport [IDA, ISO]
- nucleosome assembly [ISO]
- positive regulation of DNA metabolic process [ISO]
- positive regulation of DNA replication [ISO]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of catalytic activity [ISO]
- positive regulation of cell proliferation [IDA, IMP]
- positive regulation of cellular biosynthetic process [IDA, IMP]
- positive regulation of centrosome duplication [IGI]
- positive regulation of protein kinase activity [IDA]
- positive regulation of translation [ISO]
- protein destabilization [IMP]
- protein homooligomerization [ISO]
- protein localization [IMP, ISO]
- protein oligomerization [ISO]
- rRNA export from nucleus [IDA, IMP]
- regulation of DNA damage response, signal transduction by p53 class mediator [IGI]
- regulation of cell cycle [IMP]
- regulation of centriole replication [ISO]
- regulation of centrosome duplication [IMP]
- regulation of eIF2 alpha phosphorylation by dsRNA [ISO]
- regulation of endodeoxyribonuclease activity [ISO]
- regulation of endoribonuclease activity [ISO]
- regulation of neuron apoptotic process [ISO]
- response to stress [ISO]
- ribosomal large subunit biogenesis [IDA, IMP]
- ribosomal large subunit export from nucleus [IMP]
- ribosomal small subunit biogenesis [IDA, IMP]
- ribosomal small subunit export from nucleus [IDA]
Gene Ontology Molecular Function- DNA binding [ISO]
- NF-kappaB binding [ISO]
- RNA binding [IDA, ISO]
- Tat protein binding [ISO]
- enzyme binding [ISO]
- histone binding [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase binding [ISO]
- protein kinase inhibitor activity [ISO]
- rRNA binding [IDA]
- ribosomal large subunit binding [ISO]
- ribosomal small subunit binding [ISO]
- transcription coactivator activity [ISO]
- unfolded protein binding [ISO]
- DNA binding [ISO]
- NF-kappaB binding [ISO]
- RNA binding [IDA, ISO]
- Tat protein binding [ISO]
- enzyme binding [ISO]
- histone binding [ISO]
- phosphatidylinositol-3,4,5-trisphosphate binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein homodimerization activity [ISO]
- protein kinase binding [ISO]
- protein kinase inhibitor activity [ISO]
- rRNA binding [IDA]
- ribosomal large subunit binding [ISO]
- ribosomal small subunit binding [ISO]
- transcription coactivator activity [ISO]
- unfolded protein binding [ISO]
Gene Ontology Cellular Component
- cell [IMP]
- centrosome [IDA, ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA]
- focal adhesion [ISO]
- granular component [IDA]
- intracellular [IMP]
- large ribosomal subunit [IDA]
- membrane [ISO]
- nuclear speck [IDA]
- nucleolus [IDA, ISO]
- nucleoplasm [IDA, ISO]
- nucleus [IDA, ISO]
- ribonucleoprotein complex [ISO]
- small ribosomal subunit [IDA]
- spindle pole centrosome [ISO]
Mus musculus
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.
There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]
Cell Rep Nov. 22, 2022; 41(8);111678 [Pubmed: 36417873]
Quantitative Score
- 0.177476168 [Relative Biotinylation Score]
Throughput
- High Throughput
Additional Notes
- BioID experiment
- The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
- The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control
Curated By
- BioGRID