BAIT
CDKL5
BC038161, Stk9, RP23-213O8.2
cyclin-dependent kinase-like 5
GO Process (6)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
GRIN2B
AW490526, GluN2B, NR2B, Nmdar2b
glutamate receptor, ionotropic, NMDA2B (epsilon 2)
GO Process (35)
GO Function (17)
GO Component (14)
Gene Ontology Biological Process
- action potential [ISO]
- associative learning [ISO]
- behavioral fear response [IMP]
- behavioral response to pain [IMP]
- calcium ion transmembrane transport [IMP]
- calcium ion transport [IMP]
- cation transmembrane transport [IGI]
- cation transport [IGI]
- detection of mechanical stimulus involved in sensory perception of pain [IMP]
- embryo development [IMP]
- fear response [IMP]
- in utero embryonic development [IMP]
- ion transmembrane transport [IBA, ISO]
- ionotropic glutamate receptor signaling pathway [IGI, ISO]
- learning [IDA, IMP]
- learning or memory [TAS]
- memory [IDA, IMP, ISO]
- positive regulation of cell death [ISO]
- positive regulation of glutamate secretion [ISO]
- positive regulation of synaptic transmission [ISO]
- receptor clustering [ISO]
- regulation of MAPK cascade [ISO]
- regulation of excitatory postsynaptic membrane potential [IGI, IMP]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of neuronal synaptic plasticity [TAS]
- regulation of postsynaptic membrane potential [IMP]
- regulation of protein kinase A signaling [IMP]
- regulation of synaptic plasticity [IMP]
- response to ethanol [IMP, ISO]
- rhythmic process [ISO]
- sensory organ development [IMP]
- startle response [IGI, IMP]
- suckling behavior [IMP]
- synaptic transmission [IMP]
- synaptic transmission, glutamatergic [IBA]
Gene Ontology Molecular Function- D2 dopamine receptor binding [ISO]
- N-methyl-D-aspartate selective glutamate receptor activity [IGI, IMP, ISO]
- beta-catenin binding [ISO]
- calcium channel activity [IMP]
- cation channel activity [IGI]
- cell adhesion molecule binding [ISO]
- drug binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- glycine binding [ISO]
- interleukin-1 receptor binding [ISO]
- ionotropic glutamate receptor activity [ISO]
- ionotropic glutamate receptor binding [ISO]
- neurotransmitter binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- receptor binding [ISO]
- zinc ion binding [ISO]
- D2 dopamine receptor binding [ISO]
- N-methyl-D-aspartate selective glutamate receptor activity [IGI, IMP, ISO]
- beta-catenin binding [ISO]
- calcium channel activity [IMP]
- cation channel activity [IGI]
- cell adhesion molecule binding [ISO]
- drug binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- glycine binding [ISO]
- interleukin-1 receptor binding [ISO]
- ionotropic glutamate receptor activity [ISO]
- ionotropic glutamate receptor binding [ISO]
- neurotransmitter binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- receptor binding [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- N-methyl-D-aspartate selective glutamate receptor complex [IPI, ISO]
- Z disc [ISO]
- cell surface [IDA]
- dendritic spine [ISO]
- membrane [IDA]
- neuron projection [IDA, ISO]
- neuronal postsynaptic density [IDA]
- postsynaptic density [IDA, ISO]
- postsynaptic membrane [IDA]
- presynaptic membrane [IDA, ISO]
- synapse [IDA, ISO]
- synaptic cleft [ISO]
- synaptic vesicle [IDA]
- terminal bouton [ISO]
Mus musculus
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.
There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]
Cell Rep Nov. 22, 2022; 41(8);111678 [Pubmed: 36417873]
Quantitative Score
- 0.590527807 [Relative Biotinylation Score]
Throughput
- High Throughput
Additional Notes
- BioID experiment
- The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
- The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control
Curated By
- BioGRID