DLG4
Gene Ontology Biological Process
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [IBA, ISO]
- dendritic spine morphogenesis [IDA, ISO]
- establishment of protein localization [ISO]
- locomotory behavior [NAS]
- locomotory exploration behavior [IMP]
- negative regulation of receptor internalization [ISO]
- neuromuscular process controlling balance [IMP]
- nucleotide phosphorylation [IBA]
- positive regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of excitatory postsynaptic membrane potential [ISO]
- positive regulation of synaptic transmission [ISO]
- protein complex assembly [ISO]
- protein localization to synapse [IDA, ISO]
- receptor localization to synapse [IBA, ISO]
- regulation of N-methyl-D-aspartate selective glutamate receptor activity [ISO]
- regulation of grooming behavior [IMP]
- regulation of long-term neuronal synaptic plasticity [IGI]
- regulation of neuronal synaptic plasticity [NAS]
- response to cocaine [NAS]
- signal transduction [IBA]
- social behavior [IMP]
- synaptic vesicle maturation [IDA, IGI]
- vocalization behavior [IMP]
Gene Ontology Molecular Function- D1 dopamine receptor binding [ISO]
- P2Y1 nucleotide receptor binding [ISO]
- PDZ domain binding [ISO]
- acetylcholine receptor binding [ISO]
- beta-1 adrenergic receptor binding [ISO]
- glutamate receptor binding [ISO]
- guanylate kinase activity [IBA]
- ionotropic glutamate receptor binding [IPI, ISO]
- kinase binding [ISO]
- neurexin family protein binding [NAS]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [IPI]
- structural molecule activity [NAS]
- D1 dopamine receptor binding [ISO]
- P2Y1 nucleotide receptor binding [ISO]
- PDZ domain binding [ISO]
- acetylcholine receptor binding [ISO]
- beta-1 adrenergic receptor binding [ISO]
- glutamate receptor binding [ISO]
- guanylate kinase activity [IBA]
- ionotropic glutamate receptor binding [IPI, ISO]
- kinase binding [ISO]
- neurexin family protein binding [NAS]
- protein C-terminus binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [IPI]
- structural molecule activity [NAS]
Gene Ontology Cellular Component
- alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex [IDA]
- cell junction [IDA]
- cerebellar mossy fiber [IDA]
- cortical cytoskeleton [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- dendrite [IDA]
- dendrite cytoplasm [ISO]
- endoplasmic reticulum [IDA]
- excitatory synapse [IDA, ISO]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- ionotropic glutamate receptor complex [IDA]
- juxtaparanode region of axon [IDA, ISO]
- membrane [IDA, ISO]
- neuron projection terminus [IDA]
- neuron spine [IDA]
- neuronal postsynaptic density [IDA, ISO]
- plasma membrane [IDA, ISO]
- postsynaptic density [IDA, ISO]
- postsynaptic membrane [IDA, ISO]
- synapse [IDA, ISO]
- synaptic membrane [IDA]
- synaptic vesicle [IDA]
- voltage-gated potassium channel complex [ISO]
GRIN2A
Gene Ontology Biological Process
- action potential [ISO]
- calcium ion transmembrane transport [IMP]
- calcium ion transport [IGI, IMP]
- cation transmembrane transport [ISO]
- cation transport [ISO]
- cellular response to amino acid stimulus [ISO]
- cellular response to zinc ion [ISO]
- detection of mechanical stimulus involved in sensory perception of pain [IMP]
- directional locomotion [IGI]
- dopamine metabolic process [IMP]
- ion transmembrane transport [IGI, ISO]
- ionotropic glutamate receptor signaling pathway [IGI, ISO]
- learning [IMP]
- learning or memory [IMP]
- locomotion [IMP]
- locomotory behavior [TAS]
- memory [IMP, ISO]
- negative regulation of protein catabolic process [IGI]
- neurogenesis [IMP]
- positive regulation of apoptotic process [IGI]
- positive regulation of cell death [ISO]
- positive regulation of excitatory postsynaptic membrane potential [ISO]
- protein localization [IGI, IMP]
- protein tetramerization [ISO]
- regulation of excitatory postsynaptic membrane potential [IGI, IMP, ISO]
- regulation of ion transmembrane transport [ISO]
- regulation of long-term neuronal synaptic plasticity [ISO]
- regulation of membrane potential [IMP]
- regulation of postsynaptic membrane potential [IMP]
- regulation of sensory perception of pain [IMP]
- regulation of synaptic plasticity [IMP]
- regulation of synaptic transmission [IMP]
- response to amphetamine [IMP]
- response to drug [IMP]
- response to ethanol [IMP, ISO]
- response to wounding [IGI, IMP]
- rhythmic process [ISO]
- sensory perception of pain [IMP]
- serotonin metabolic process [IMP]
- sleep [IMP]
- startle response [IGI, IMP]
- synaptic transmission [IMP, ISO]
- synaptic transmission, glutamatergic [IBA]
- visual learning [IMP]
Gene Ontology Molecular Function- ATPase binding [ISO]
- N-methyl-D-aspartate selective glutamate receptor activity [IGI, IMP, ISO]
- calcium channel activity [IGI, IMP]
- cation channel activity [IMP, ISO]
- cell adhesion molecule binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- glutamate binding [ISO]
- glutamate receptor binding [ISO]
- ionotropic glutamate receptor activity [ISO]
- neurotransmitter binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein dimerization activity [ISO]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [ISO]
- voltage-gated cation channel activity [ISO]
- zinc ion binding [ISO]
- ATPase binding [ISO]
- N-methyl-D-aspartate selective glutamate receptor activity [IGI, IMP, ISO]
- calcium channel activity [IGI, IMP]
- cation channel activity [IMP, ISO]
- cell adhesion molecule binding [ISO]
- extracellular-glutamate-gated ion channel activity [IBA, ISO]
- glutamate binding [ISO]
- glutamate receptor binding [ISO]
- ionotropic glutamate receptor activity [ISO]
- neurotransmitter binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein dimerization activity [ISO]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- receptor binding [ISO]
- scaffold protein binding [ISO]
- voltage-gated cation channel activity [ISO]
- zinc ion binding [ISO]
Gene Ontology Cellular Component
- N-methyl-D-aspartate selective glutamate receptor complex [IPI, ISO]
- cell surface [IDA]
- dendritic spine [ISA]
- endoplasmic reticulum [IDA]
- membrane [IDA]
- neuron projection [IDA, ISO]
- neuronal postsynaptic density [IDA]
- postsynaptic density [IDA, ISO]
- postsynaptic membrane [IDA]
- presynaptic membrane [IDA, ISO]
- synapse [IDA]
- synaptic vesicle [IDA]
- terminal bouton [ISO]
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.
There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]
Quantitative Score
- 0.518451926 [Relative Biotinylation Score]
Throughput
- High Throughput
Additional Notes
- BioID experiment
- The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
- The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DLG4 GRIN2A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| DLG4 GRIN2A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low/High | - | BioGRID | 2333701 | |
| DLG4 GRIN2A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| GRIN2A DLG4 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| DLG4 GRIN2A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| DLG4 GRIN2A | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
| DLG4 GRIN2A | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID