An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.

Murtaza N, Cheng AA, Brown CO, Meka DP, Hong S, Uy JA, El-Hajjar J, Pipko N, Unda BK, Schwanke B, Xing S, Thiruvahindrapuram B, Engchuan W, Trost B, Deneault E, Calderon de Anda F, Doble BW, Ellis J, Anagnostou E, Bader GD, Scherer SW, Lu Y, Singh KK

There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]

Cell Rep Nov. 22, 2022; 41(8);111678 [Pubmed: 36417873]

Quantitative Score

0.441931796 [Relative Biotinylation Score]

Throughput

High Throughput

Additional Notes

BioID experiment
The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DLG4 SH3KBP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Unda BK (2023)	Low	-	BioGRID	-
DLG4 SH3KBP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Shimokawa N (2010)	Low	-	BioGRID	-
SH3KBP1 DLG4	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Shimokawa N (2010)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

DLG4

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

SH3KBP1