BAIT
GRIA2
GluA2, GluR-B, Glur-2, Glur2
glutamate receptor, ionotropic, AMPA2 (alpha 2)
GO Process (10)
GO Function (9)
GO Component (22)
Gene Ontology Biological Process
- establishment of protein localization [ISO]
- ion transmembrane transport [IBA]
- ionotropic glutamate receptor signaling pathway [IBA, ISO]
- positive regulation of synaptic transmission [ISO]
- protein tetramerization [ISO]
- receptor internalization [ISO]
- regulation of receptor recycling [ISO]
- regulation of synaptic transmission, glutamatergic [ISO]
- synaptic transmission [IDA, ISO]
- synaptic transmission, glutamatergic [IBA]
Gene Ontology Molecular Function- PDZ domain binding [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IBA, ISO]
- extracellular-glutamate-gated ion channel activity [IDA]
- identical protein binding [ISO]
- ionotropic glutamate receptor activity [IDA, ISO]
- kainate selective glutamate receptor activity [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor activity [ISO]
- PDZ domain binding [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IBA, ISO]
- extracellular-glutamate-gated ion channel activity [IDA]
- identical protein binding [ISO]
- ionotropic glutamate receptor activity [IDA, ISO]
- kainate selective glutamate receptor activity [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- receptor activity [ISO]
Gene Ontology Cellular Component
- alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex [IDA, ISO]
- asymmetric synapse [ISO]
- cell surface [ISO]
- dendrite [IBA, ISO]
- dendrite cytoplasm [ISO]
- dendritic shaft [ISO]
- dendritic spine [ISO]
- endoplasmic reticulum [IDA]
- growth cone [ISO]
- integral component of plasma membrane [ISO]
- membrane [IDA]
- neuron projection [IDA]
- neuronal cell body [ISO]
- perikaryon [ISO]
- postsynaptic density [ISO]
- postsynaptic membrane [IBA, IDA]
- presynaptic membrane [ISO]
- protein complex [ISO]
- synapse [IDA, ISO]
- synaptic vesicle [IDA]
- synaptic vesicle membrane [ISO]
- terminal bouton [ISO]
Mus musculus
PREY
RYR2
9330127I20Rik, RYR-2
ryanodine receptor 2, cardiac
GO Process (40)
GO Function (16)
GO Component (21)
Gene Ontology Biological Process
- BMP signaling pathway [IDA]
- Purkinje myocyte to ventricular cardiac muscle cell signaling [IMP]
- calcium ion transmembrane transport [IDA, IMP, ISO]
- calcium ion transport [IDA, IMP, IPI, ISO]
- calcium ion transport into cytosol [ISO]
- calcium-mediated signaling [IMP]
- calcium-mediated signaling using intracellular calcium source [ISO]
- canonical Wnt signaling pathway [IDA]
- cardiac muscle contraction [ISO]
- cardiac muscle hypertrophy [IMP]
- cellular calcium ion homeostasis [IMP]
- cellular response to caffeine [IDA, ISO]
- cytosolic calcium ion homeostasis [ISO]
- cytosolic calcium ion transport [IMP]
- detection of calcium ion [ISO]
- embryonic heart tube morphogenesis [IMP]
- establishment of protein localization to endoplasmic reticulum [ISO]
- ion transmembrane transport [ISO]
- left ventricular cardiac muscle tissue morphogenesis [IMP]
- manganese ion transmembrane transport [ISO]
- negative regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of calcium-transporting ATPase activity [ISO]
- positive regulation of heart rate [IMP]
- positive regulation of ryanodine-sensitive calcium-release channel activity by adrenergic receptor signaling pathway involved in positive regulation of cardiac muscle contraction [IMP]
- positive regulation of sequestering of calcium ion [ISO]
- positive regulation of the force of heart contraction [ISO]
- regulation of cardiac muscle contraction [ISO]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [IGI]
- regulation of heart rate [IMP, ISO]
- release of sequestered calcium ion into cytosol [ISO]
- release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [IDA, IMP, ISO]
- response to caffeine [IDA, ISO]
- response to calcium ion [ISO]
- response to hypoxia [IDA]
- response to magnesium ion [ISO]
- response to muscle stretch [ISO]
- response to redox state [ISO]
- sarcoplasmic reticulum calcium ion transport [ISO]
- type B pancreatic cell apoptotic process [IMP, ISO]
- ventricular cardiac muscle cell action potential [IMP]
Gene Ontology Molecular Function- calcium channel activity [IDA]
- calcium-induced calcium release activity [ISO]
- calcium-release channel activity [ISO]
- calmodulin binding [IDA, ISO]
- enzyme binding [IPI, ISO]
- identical protein binding [ISO]
- intracellular ligand-gated calcium channel activity [IDA]
- organic cyclic compound binding [IPI]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase binding [IPI]
- protein self-association [IMP]
- ryanodine-sensitive calcium-release channel activity [IDA, ISO]
- scaffold protein binding [ISO]
- suramin binding [ISO]
- calcium channel activity [IDA]
- calcium-induced calcium release activity [ISO]
- calcium-release channel activity [ISO]
- calmodulin binding [IDA, ISO]
- enzyme binding [IPI, ISO]
- identical protein binding [ISO]
- intracellular ligand-gated calcium channel activity [IDA]
- organic cyclic compound binding [IPI]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase A regulatory subunit binding [ISO]
- protein kinase binding [IPI]
- protein self-association [IMP]
- ryanodine-sensitive calcium-release channel activity [IDA, ISO]
- scaffold protein binding [ISO]
- suramin binding [ISO]
Gene Ontology Cellular Component
- A band [ISO]
- Z disc [IDA, ISO]
- calcium channel complex [IDA, ISO]
- cell [IMP]
- cytoplasm [ISO]
- cytosol [IMP]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- extrinsic component of cytoplasmic side of plasma membrane [ISO]
- integral component of membrane [IC]
- intracellular [IGI]
- membrane [IDA, ISO]
- neuron projection [ISO]
- nuclear envelope [ISO]
- plasma membrane [ISO]
- protein complex [ISO]
- sarcolemma [ISO]
- sarcomere [IDA]
- sarcoplasmic reticulum [IDA, ISO]
- sarcoplasmic reticulum membrane [IDA, ISO]
- smooth endoplasmic reticulum [IDA]
Mus musculus
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.
There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]
Cell Rep Nov. 22, 2022; 41(8);111678 [Pubmed: 36417873]
Quantitative Score
- 0.262645456 [Relative Biotinylation Score]
Throughput
- High Throughput
Additional Notes
- BioID experiment
- The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
- The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control
Curated By
- BioGRID