BAIT
SHANK3
AI841104, SHANK3c-3, SHANK3c-4, Shank3b
SH3/ankyrin domain gene 3
GO Process (33)
GO Function (9)
GO Component (10)
Gene Ontology Biological Process
- MAPK cascade [IGI]
- N-methyl-D-aspartate receptor clustering [IMP]
- adult behavior [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [IMP]
- brain morphogenesis [IMP]
- dendritic spine morphogenesis [IMP]
- embryonic epithelial tube formation [IGI]
- guanylate kinase-associated protein clustering [IMP]
- learning [IMP, ISO]
- locomotory exploration behavior [IMP]
- memory [IMP]
- negative regulation of actin filament bundle assembly [IDA]
- negative regulation of cell volume [IMP]
- neuromuscular process controlling balance [IMP]
- positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [IMP]
- positive regulation of dendritic spine development [IMP]
- positive regulation of excitatory postsynaptic membrane potential [IMP]
- positive regulation of glutamate receptor signaling pathway [IMP, ISO]
- positive regulation of long-term neuronal synaptic plasticity [IMP]
- positive regulation of synapse structural plasticity [IMP]
- positive regulation of synaptic transmission, glutamatergic [IMP]
- postsynaptic density assembly [IMP]
- protein oligomerization [ISO]
- regulation of behavioral fear response [IMP]
- regulation of dendritic spine morphogenesis [IMP]
- regulation of grooming behavior [IMP]
- regulation of long term synaptic depression [IMP]
- regulation of long-term synaptic potentiation [IMP]
- social behavior [IMP, ISO]
- striatal medium spiny neuron differentiation [IMP]
- synapse assembly [IMP]
- vocal learning [ISO]
- vocalization behavior [IMP, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ANK3
2900054D09Rik, AI314020, Ank-3, AnkG, Ankyrin-3, Ankyrin-G
ankyrin 3, epithelial
GO Process (23)
GO Function (7)
GO Component (24)
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISO]
- axon guidance [IMP]
- axonogenesis [IMP]
- establishment of protein localization [ISO]
- maintenance of protein location in plasma membrane [ISO]
- membrane assembly [ISO]
- mitotic cytokinesis [ISO]
- neuronal action potential [IMP]
- plasma membrane organization [ISO]
- positive regulation of action potential [TAS]
- positive regulation of cation channel activity [ISO]
- positive regulation of cell communication by electrical coupling [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of homotypic cell-cell adhesion [ISO]
- positive regulation of membrane depolarization during cardiac muscle cell action potential [ISO]
- positive regulation of membrane potential [ISO]
- positive regulation of protein targeting to membrane [ISO]
- positive regulation of sodium ion transmembrane transporter activity [ISO]
- positive regulation of sodium ion transport [ISO]
- protein localization to plasma membrane [IDA, IMP, ISO]
- protein targeting to plasma membrane [IMP, ISO]
- regulation of potassium ion transport [ISO]
- synapse organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [ISO]
- Z disc [ISO]
- axon [IDA]
- axon initial segment [IDA, ISO]
- basal plasma membrane [ISO]
- basolateral plasma membrane [ISO]
- cell [IMP]
- cell surface [ISO]
- dendrite [ISO]
- intercalated disc [IDA, ISO]
- intracellular [IMP]
- lateral plasma membrane [ISO]
- membrane [IDA]
- neuromuscular junction [ISO]
- neuron projection [ISO]
- node of Ranvier [IDA, ISO]
- paranode region of axon [IDA]
- plasma membrane [ISO]
- postsynaptic membrane [ISO]
- sarcolemma [ISO]
- sarcoplasmic reticulum [ISO]
- spectrin-associated cytoskeleton [ISO]
- synapse [IDA]
- tight junction [ISO]
Mus musculus
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.
There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]
Cell Rep Nov. 22, 2022; 41(8);111678 [Pubmed: 36417873]
Quantitative Score
- 0.280423794 [Relative Biotinylation Score]
Throughput
- High Throughput
Additional Notes
- BioID experiment
- The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
- The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SHANK3 ANK3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| SHANK3 ANK3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2334000 |
Curated By
- BioGRID