BAIT
SPAST
Spg4, mKIAA1083
spastin
GO Process (8)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
RTN4
1110020G17Rik, AA407876, AA409940, AA960376, ASY, C130026I10Rik, NOGO, NSP-CL, NgA, Nogo-A, Nogo-B, Nogo-C, mKIAA0886, mKIAA4153, RP23-176O5.4
reticulon 4
GO Process (19)
GO Function (3)
GO Component (13)
Gene Ontology Biological Process
- angiogenesis [IMP]
- axonal fasciculation [IMP, ISO]
- cardiac epithelial to mesenchymal transition [IMP]
- cerebral cortex radial glia guided migration [IMP]
- endoplasmic reticulum tubular network organization [ISO]
- negative regulation of axon extension [IMP, ISO, ISS]
- negative regulation of axonogenesis [ISO]
- negative regulation of cell growth [ISO]
- negative regulation of neuron differentiation [ISO]
- negative regulation of neuron projection development [ISO]
- negative regulation of neuron projection regeneration [ISO]
- nervous system development [IDA]
- positive regulation of dopamine secretion [ISO]
- positive regulation of glial cell differentiation [ISO]
- regulation of axon regeneration [ISO]
- regulation of branching morphogenesis of a nerve [IMP, ISO]
- regulation of cell migration [ISO]
- regulation of nervous system development [IMP]
- regulation of sensory perception of pain [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell projection [IDA]
- cytoplasm [ISO]
- endoplasmic reticulum [IDA, ISO]
- extracellular vesicular exosome [ISO]
- integral component of endoplasmic reticulum membrane [ISO, ISS]
- intracellular [ISO]
- myelin sheath [ISO]
- neuron projection [ISO]
- neuronal cell body [IDA, ISO]
- neuronal postsynaptic density [IDA]
- nuclear envelope [ISO, ISS]
- plasma membrane [ISO]
- protein complex [ISO]
Mus musculus
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.
There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]
Cell Rep Nov. 22, 2022; 41(8);111678 [Pubmed: 36417873]
Quantitative Score
- 0.240167311 [Relative Biotinylation Score]
Throughput
- High Throughput
Additional Notes
- BioID experiment
- The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
- The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control
Curated By
- BioGRID