Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies.

Murtaza N, Cheng AA, Brown CO, Meka DP, Hong S, Uy JA, El-Hajjar J, Pipko N, Unda BK, Schwanke B, Xing S, Thiruvahindrapuram B, Engchuan W, Trost B, Deneault E, Calderon de Anda F, Doble BW, Ellis J, Anagnostou E, Bader GD, Scherer SW, Lu Y, Singh KK

There are hundreds of risk genes associated with autism spectrum disorder (ASD), but signaling networks at the protein level remain unexplored. We use neuron-specific proximity-labeling proteomics (BioID2) to identify protein-protein interaction (PPI) networks for 41 ASD risk genes. Neuron-specific PPI networks, including synaptic transmission proteins, are disrupted by de novo missense variants. The PPI network map reveals convergent pathways, including ... [more]

Cell Rep Nov. 22, 2022; 41(8);111678 [Pubmed: 36417873]

Quantitative Score

  • 0.417951276 [Relative Biotinylation Score]

Throughput

  • High Throughput

Additional Notes

  • BioID experiment
  • The scores are relative biotinylation enrichment scores (calculated as a percentage of the abundance fold change of the highest biotinylated prey protein)
  • The statistical cut-offs for the prey proteins are based on adjusted t-test p-value and significance B scores of less than 0.05, by comparing average abundances of prey proteins between the Bait-BioID samples and the luciferase-BioID control

Curated By

  • BioGRID