Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Nondegradable ubiquitinated ATG9A organizes Golgi integrity and dynamics upon stresses.

Luo Q, Liu Q, Cheng H, Wang J, Zhao T, Zhang J, Mu C, Meng Y, Chen L, Zhou C, Lei H, Yang J, Chen G, Li Y, Pan L, Chen Q, Zhu Y

ATG9A is a highly conserved membrane protein required for autophagy initiation. It is trafficked from the trans-Golgi network (TGN) to the phagophore to act as a membrane source for autophagosome expansion. Here, we show that ATG9A is not just a passenger protein in the TGN but rather works in concert with GRASP55, a stacking factor for Golgi structure, to organize ... [more]

Cell Rep Aug. 16, 2022; 40(7);111195 [Pubmed: 35977480]

Throughput

  • Low Throughput

Additional Notes

  • Source of MARCH9 not clear

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MARCH9 ATG9A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.7955BioGRID
2269055
MARCH9 ATG9A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.7555BioGRID
3127726

Curated By

  • BioGRID