BAIT

STU1

L000002138, YBL034C
Component of the mitotic spindle; binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

CDC20

PAC5, ubiquitin-protein transferase activating protein CDC20, L000000259, YGL116W
Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
UPS Project
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast.

Chiroli E, Rancati G, Catusi I, Lucchini G, Piatti S

The spindle assembly checkpoint (SAC) is an evolutionarily conserved surveillance mechanism that delays anaphase onset and mitotic exit in response to the lack of kinetochore attachment. The target of the SAC is the E3 ubiquitin ligase anaphase-promoting complex (APC) bound to its Cdc20 activator. The Cdc20/APC complex is in turn required for sister chromatid separation and mitotic exit through ubiquitin-mediated ... [more]

Mol. Biol. Cell May. 01, 2009; 20(10);2626-37 [Pubmed: 19339280]

Throughput

  • Low Throughput

Ontology Terms

  • chromosome segregation (APO:0000208)

Additional Notes

  • genetic complex
  • mutation of CDC20 blocks the partial rescue of chromatid separation that is seen when MAD2 is deleted in a STU1 mutant background
  • mutation of CDC20 blocks the partial rescue of chromatid separation that is seen when MAD3 is deleted in a STU1 mutant background

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
STU1 CDC20
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2557BioGRID
1919202
STU1 CDC20
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
350182

Curated By

  • BioGRID