BAIT

PLEKHM1

AP162, B2, OPTB6
pleckstrin homology domain containing, family M (with RUN domain) member 1
Autophagy Project
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

VPS11

END1, PEP5, RNF108, hVPS11, PP3476
vacuolar protein sorting 11 homolog (S. cerevisiae)
Autophagy Project
UPS Project
GO Process (0)
GO Function (2)
GO Component (6)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The endolysosomal adaptor PLEKHM1 is a direct target for both mTOR and MAPK pathways.

Gubas A, Karantanou C, Popovic D, Tascher G, Hoffmann ME, Platzek A, Dawe N, Dikic I, Krause DS, McEwan DG

The lysosome is a cellular signalling hub at the point of convergence of endocytic and autophagic pathways, where the contents are degraded and recycled. Pleckstrin homology domain-containing family member 1 (PLEKHM1) acts as an adaptor to facilitate the fusion of endocytic and autophagic vesicles with the lysosome. However, it is unclear how PLEKHM1 function at the lysosome is controlled. Herein, ... [more]

FEBS Lett Apr. 01, 2021; 595(7);864-880 [Pubmed: 33452816]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PLEKHM1 VPS11
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
PLEKHM1 VPS11
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID