Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The OTUD6B-LIN28B-MYC axis determines the proliferative state in multiple myeloma.

Paulmann C, Spallek R, Karpiuk O, Heider M, Schaeffer I, Zecha J, Klaeger S, Walzik M, Oellinger R, Engleitner T, Wirth M, Keller U, Kroenke J, Rudelius M, Kossatz S, Rad R, Kuster B, Bassermann F

Deubiquitylases (DUBs) are therapeutically amenable components of the ubiquitin machinery that stabilize substrate proteins. Their inhibition can destabilize oncoproteins that may otherwise be undruggable. Here, we screened for DUB vulnerabilities in multiple myeloma, an incurable malignancy with dependency on the ubiquitin proteasome system and identified OTUD6B as an oncogene that drives the G1/S-transition. LIN28B, a suppressor of microRNA biogenesis, is ... [more]

EMBO J Oct. 17, 2022; 41(20);e110871 [Pubmed: 36059274]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
OTUD6B RPLP0
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
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Curated By

  • BioGRID