Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Functional redundancy among Polycomb complexes in maintaining the pluripotent state of embryonic stem cells.

Zhu Y, Dong L, Wang C, Hao K, Wang J, Zhao L, Xu L, Xia Y, Jiang Q, Qin J

Polycomb group proteins assemble into multi-protein complexes, known as Polycomb repressive complexes 1 and 2 (PRC1 and PRC2), that guide cell fate decisions during embryonic development. PRC1 forms an array of biochemically distinct canonical PRC1 (cPRC1) or non-canonical PRC1 (ncPRC1) complexes characterized by the mutually exclusive presence of PCGF (PCGF1-PCGF6) paralog subunit; however, whether each one of these subcomplexes fulfills ... [more]

Stem Cell Reports May. 10, 2022; 17(5);1198-1214 [Pubmed: 35364009]

Throughput

  • Low Throughput

Ontology Terms

  • biological process: stem cell proliferation (GO:0072089)

Additional Notes

  • CRISPR method
  • deletion of pcgf1/2/3/4/5 combined with conditional removal of pcgf6 resulted in decreased ESC proliferation combined with loss of pluripotency markers and differentiated morphology
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BMI1 PCGF6
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High48.9019BioGRID
2942720
PCGF6 BMI1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID