BAIT

RPB11

BcDNA:GM15177, CG6840, Dmel\CG6840, Dmel_CG6840
CG6840 gene product from transcript CG6840-RA
GO Process (2)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Drosophila melanogaster
PREY

RPII33

152117_at, BG:DS00941.10, CG7885, D-rpII33, Dmel\CG7885, Pol II, Pol II RPII33, PolII, PolIIo, RNA Pol II, RNA polII, RNAP, RPB3, Rbp3, Rpll33, br17, l(2)34Dg, l(2)br17, l(2)k05605, l34Dg, Dmel_CG7885
RNA polymerase II 33kD subunit
Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein interaction map of Drosophila melanogaster.

Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B, Li Y, Hao YL, Ooi CE, Godwin B, Vitols E, Vijayadamodar G, Pochart P, Machineni H, Welsh M, Kong Y, Zerhusen B, Malcolm R, Varrone Z, Collis A, Minto M, Burgess S, McDaniel L, Stimpson E, Spriggs F, Williams J, Neurath K, Ioime N, Agee M, Voss E, Furtak K, Renzulli R, Aanensen N, Carrolla S, Bickelhaupt E, Lazovatsky Y, DaSilva A, Zhong J, Stanyon CA, Finley RL, White KP, Braverman M, Jarvie T, Gold S, Leach M, Knight J, Shimkets RA, McKenna MP, Chant J, Rothberg JM

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence ... [more]

Science Dec. 05, 2003; 302(5651);1727-36 [Pubmed: 14605208]

Throughput

  • High Throughput

Curated By

  • BioGRID