An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Physical and functional interactome atlas of human receptor tyrosine kinases.

Salokas K, Liu X, Oehman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M

Much cell-to-cell communication is facilitated by cell surface receptor tyrosine kinases (RTKs). These proteins phosphorylate their downstream cytoplasmic substrates in response to stimuli such as growth factors. Despite their central roles, the functions of many RTKs are still poorly understood. To resolve the lack of systematic knowledge, we apply three complementary methods to map the molecular context and substrate profiles ... [more]

EMBO Rep Jun. 07, 2022; 23(6);e54041 [Pubmed: 35384245]

Quantitative Score

0.03 [BFDR Score]

Throughput

High Throughput

Additional Notes

Proximity Label-MS was carried out to identify high confidence protein interactors with a BFDR of less than or equal to 0.05 (BFDR score reported)

Curated By

BioGRID

Interaction Summary

RYK

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein tyrosine kinase activity [IDA]transmembrane receptor protein tyrosine kinase activity [TAS]transmembrane signaling receptor activity [IDA]

Gene Ontology Cellular Component

DST

Gene Ontology Biological Process

Gene Ontology Molecular Function

integrin binding [IPI]microtubule plus-end binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]protein homodimerization activity [IDA]