Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein

Kuhn CC, Basnet N, Bodakuntla S, Alvarez- Brecht P, Nichols S, Martinez-Sanchez A, Agostini L, Soh Y-M, Takagi J, Biertumpfel C, Mizuno N

SARS-CoV-2 is a novel coronavirus responsible for the COVID-19 pandemic. Its high pathogenicity is due to SARS-CoV-2 spike protein (S protein) contacting host-cell receptors. A critical hallmark of COVID-19 is the occurrence of coagulopathies. Here, we report the direct observation of the interactions between S protein and platelets. Live imaging showed that the S protein triggers platelets to deform dynamically, ... [more]

Status: Preliminary Report

Throughput

  • Low Throughput

Additional Notes

  • Hit is Integrin A5B1 (ITGA5 and ITGB1)

Curated By

  • BioGRID