Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

C16orf72/HAPSTR1 is a molecular rheostat in an integrated network of stress response pathways.

Amici DR, Ansel DJ, Metz KA, Smith RS, Phoumyvong CM, Gayatri S, Chamera T, Edwards SL, O'Hara BP, Srivastava S, Brockway S, Takagishi SR, Cho BK, Goo YA, Kelleher NL, Ben-Sahra I, Foltz DR, Li J, Mendillo ML

All cells contain specialized signaling pathways that enable adaptation to specific molecular stressors. Yet, whether these pathways are centrally regulated in complex physiological stress states remains unclear. Using genome-scale fitness screening data, we quantified the stress phenotype of 739 cancer cell lines, each representing a unique combination of intrinsic tumor stresses. Integrating dependency and stress perturbation transcriptomic data, we illuminated ... [more]

Proc Natl Acad Sci U S A Dec. 05, 2021; 119(27);e2111262119 [Pubmed: 35776542]

Throughput

  • High Throughput

Additional Notes

  • Interactions were determined to be high confidence if the log2FC spectra in the C16orf72 vs GFP IP-MS experiment was greater than 3 (log2FC > 3).

Curated By

  • BioGRID