BAIT

IFNB1

IFB, IFF, IFNB, RP11-113D19.1
interferon, beta 1, fibroblast
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interferon-? Activity Is Affected by S100B Protein.

Kazakov AS, Sofin AD, Avkhacheva NV, Deryusheva EI, Rastrygina VA, Permyakova ME, Uversky VN, Permyakov EA, Permyakov SE

Interferon-? (IFN-?) is a pleiotropic cytokine secreted in response to various pathological conditions and is clinically used for therapy of multiple sclerosis. Its application for treatment of cancer, infections and pulmonary diseases is limited by incomplete understanding of regulatory mechanisms of its functioning. Recently, we reported that IFN-? activity is affected by interactions with S100A1, S100A4, S100A6, and S100P proteins, ... [more]

Int J Mol Sci Feb. 11, 2022; 23(4); [Pubmed: 35216109]

Throughput

  • Low Throughput

Additional Notes

  • Cell viability tests
  • Recombinant proteins expressed in CHO (InteractorA) and E. coli (InteractorB)

Curated By

  • BioGRID