BAIT

IFNB1

IFB, IFF, IFNB, RP11-113D19.1
interferon, beta 1, fibroblast
Homo sapiens
PREY

S100A4

18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
S100 calcium binding protein A4
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interferon Beta Activity Is Modulated via Binding of Specific S100 Proteins.

Kazakov AS, Sofin AD, Avkhacheva NV, Denesyuk AI, Deryusheva EI, Rastrygina VA, Sokolov AS, Permyakova ME, Litus EA, Uversky VN, Permyakov EA, Permyakov SE

Interferon-? (IFN-?) is a pleiotropic cytokine used for therapy of multiple sclerosis, which is also effective in suppression of viral and bacterial infections and cancer. Recently, we reported a highly specific interaction between IFN-? and S100P lowering IFN-? cytotoxicity to cancer cells (Int J Biol Macromol. 2020; 143: 633-639). S100P is a member of large family of multifunctional Ca2+-binding proteins ... [more]

Int J Mol Sci Dec. 13, 2020; 21(24); [Pubmed: 33322098]

Throughput

  • Low Throughput

Additional Notes

  • Cell viability tests

Curated By

  • BioGRID