An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The in vivo Interaction Landscape of Histones H3.1 and H3.3.

Siddaway R, Milos S, Coyaud E, Yun HY, Morcos SM, Pajovic S, Campos EI, Raught B, Hawkins C

Chromatin structure, transcription, DNA replication, and repair are regulated via locus-specific incorporation of histone variants and posttranslational modifications that guide effector chromatin-binding proteins. Here we report unbiased, quantitative interactomes for the replication-coupled (H3.1) and replication-independent (H3.3) histone H3 variants based on BioID proximity labeling, which allows interactions in intact, living cells to be detected. Along with a significant proportion of ... [more]

Mol Cell Proteomics Oct. 01, 2022; 21(10);100411 [Pubmed: 36089195]

Throughput

High Throughput

Additional Notes

BioID
FDR less than or equal to 1%

Curated By

BioGRID

Interaction Summary

HIST1H3C

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

ARID3B