A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The eIF3 interactome reveals the translasome, a supercomplex linking protein synthesis and degradation machineries.

Sha Z, Brill LM, Cabrera R, Kleifeld O, Scheliga JS, Glickman MH, Chang EC, Wolf DA

eIF3 promotes translation initiation, but relatively little is known about its full range of activities in the cell. Here, we employed affinity purification and highly sensitive LC-MS/MS to decipher the fission yeast eIF3 interactome, which was found to contain 230 proteins. eIF3 assembles into a large supercomplex, the translasome, which contains elongation factors, tRNA synthetases, 40S and 60S ribosomal proteins, ... [more]

Mol. Cell Oct. 09, 2009; 36(1);141-52 [Pubmed: 19818717]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Curated By

BioGRID

Interaction Summary

KAP123

Gene Ontology Biological Process

Gene Ontology Cellular Component

SAL3