Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

TRF2-mediated telomere protection is dispensable in pluripotent stem cells.

Markiewicz-Potoczny M, Lobanova A, Loeb AM, Kirak O, Olbrich T, Ruiz S, Lazzerini Denchi E

In mammals, telomere protection is mediated by the essential protein TRF2, which binds chromosome ends and ensures genome integrity1,2. TRF2 depletion results in end-to-end chromosome fusions in all cell types that have been tested so far. Here we find that TRF2 is dispensable for the proliferation and survival of mouse embryonic stem (ES) cells. Trf2-/- (also known as Terf2) ES ... [more]

Nature Jan. 01, 2021; 589(7840);110-115 [Pubmed: 33239785]

Throughput

Low Throughput

Ontology Terms

phenotype: viability (PATO:0000169)

Additional Notes

CRISPR GI screen
Cell Line: ES Trf2-/-
Experimental Setup: Timecourse, 14 days
GIST: A-phenotypic negative genetic interaction
Library: Brie
Significance Threshold: genes that are essential (Beta score < -0.75) for TRF2-null ES cells but do not affect the growth of TRF2-proficient cells (Beta score > -0.25)

Curated By

BioGRID

Interaction Summary

TERF2

Gene Ontology Biological Process

Gene Ontology Molecular Function

double-stranded telomeric DNA binding [IMP]protein C-terminus binding [ISO]protein binding [IPI]protein homodimerization activity [IPI, ISO]telomeric DNA binding [ISO]

Gene Ontology Cellular Component

TSEN2