TERF2
Gene Ontology Biological Process
- in utero embryonic development [IMP]
- negative regulation of telomere maintenance [ISO]
- positive regulation of telomere maintenance [IDA, IMP]
- protection from non-homologous end joining at telomere [IDA, IMP, ISO]
- protein localization to chromosome, telomeric region [ISO]
- telomere capping [ISO]
- telomere maintenance [IMP, ISO]
- telomere maintenance via telomere shortening [IBA]
- telomeric loop formation [IBA, ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
POT1B
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
TRF2-mediated telomere protection is dispensable in pluripotent stem cells.
In mammals, telomere protection is mediated by the essential protein TRF2, which binds chromosome ends and ensures genome integrity1,2. TRF2 depletion results in end-to-end chromosome fusions in all cell types that have been tested so far. Here we find that TRF2 is dispensable for the proliferation and survival of mouse embryonic stem (ES) cells. Trf2-/- (also known as Terf2) ES ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: viability (PATO:0000169)
Additional Notes
- CRISPR GI screen
- Cell Line: ES Trf2-/-
- Experimental Setup: Timecourse, 14 days
- GIST: A-phenotypic negative genetic interaction
- Library: Brie
- Significance Threshold: genes that are essential (Beta score < -0.75) for TRF2-null ES cells but do not affect the growth of TRF2-proficient cells (Beta score > -0.25)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TERF2 POT1B | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 3515398 | |
| TERF2 POT1B | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 3515400 |
Curated By
- BioGRID