BAIT

CDC36

DNA19, NOT2, CCR4-NOT core subunit CDC36, L000000272, YDL165W
Component of the CCR4-NOT core complex; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs through deadenylation; basal transcription factor
Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The CCR4-NOT complex physically and functionally interacts with TRAMP and the nuclear exosome.

Azzouz N, Panasenko OO, Colau G, Collart MA

BACKGROUND: Ccr4-Not is a highly conserved multi-protein complex consisting in yeast of 9 subunits, including Not5 and the major yeast deadenylase Ccr4. It has been connected functionally in the nucleus to transcription by RNA polymerase II and in the cytoplasm to mRNA degradation. However, there has been no evidence so far that this complex is important for RNA degradation in ... [more]

PLoS ONE Aug. 27, 2009; 4(8);e6760 [Pubmed: 19707589]

Throughput

  • Low Throughput

Curated By

  • BioGRID