BAIT

SPP382

CCF8, NTR1, YLR424W
Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
Saccharomyces cerevisiae (S288c)
PREY

PRP38

L000001510, YGR075C
Unique component of the U4/U6.U5 tri-snRNP particle; tri-snRNP is required for conformational changes which result in the catalytic activation of the spliceosome; dispensable for spliceosome assembly
GO Process (1)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Spp382p interacts with multiple yeast splicing factors, including possible regulators of Prp43 DExD/H-Box protein function.

Pandit S, Paul S, Zhang L, Chen M, Durbin N, Harrison SM, Rymond BC

Prp43p catalyzes essential steps in pre-mRNA splicing and rRNA biogenesis. In splicing, Spp382p stimulates the Prp43p helicase to dissociate the postcatalytic spliceosome and, in some way, to maintain the integrity of the spliceosome assembly. Here we present a dosage interference assay to identify Spp382p-interacting factors by screening for genes that when overexpressed specifically inhibit the growth of a conditional lethal ... [more]

Genetics Sep. 01, 2009; 183(1);195-206 [Pubmed: 19581443]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: viability (APO:0000111)
  • phenotype: inviable (APO:0000112)

Additional Notes

  • CWC23 overexpression is lethal in a PRP38/SPP382 double mutant
  • DHH1 overexpression is lethal in a PRP38/SPP382 double mutant
  • MRP13 overexpression is lethal in a PRP38/SPP382 double mutant
  • MRP13 overexpression is lethal in a PRP38/SPP382 double mutant at high temp
  • Overexpression of SQS1 is lethal in a PRP38/SPP382 double mutant
  • PRP39 overexpression is lethal in a PRP38/SPP382 double mutant
  • PRP43 overexpression is lethal in a PRP38/SPP382 double mutant
  • YDR230W overexpression is lethal in a PRP38/SPP382 double mutant
  • YML6 overexpression is lethal in a PRP38/SPP382 double mutant
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRP38 SPP382
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
644888
PRP38 SPP382
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
205091
PRP38 SPP382
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
351797

Curated By

  • BioGRID